Development of a New Approach for Screening Combinatorial Libraries Using MALDI-TOF-MS and HPLC-ESI-MS/MS

Identification of mammalian cell lines using MALDI-TOF and LC-ESI-MS/MS mass spectrometry.

Direct mass spectrometric analysis of complex biological samples is becoming an increasingly useful technique in the field of proteomics. Matrix-assisted laser desorption/ionization mass spectroscopy (MALDI-MS) is a rapid and sensitive analytical tool well suited for obtaining molecular weights of peptides and proteins from complex samples. Here, a fast and simple approach to cellular protein p...

Identification of chemical constituents in cuscuta chinensis using HPLC-ESI/Q-TOF MS/MS

AIM: To identify the chemical constituents in Cuscuta Chinensis. METHODS: A High-performance liquid chromatography (HPLC) method combined with quadrupole-time-of-flight-mass spectrometry (QTOF-MS) has been developed for precise separation and structural identification of components in Cuscuta Chinensis. Mass spectrometry was performed in reflective time-of-flight using electron spraying ionizat...

A Highly Accurate Mass Profiling Approach to Protein Biomarker Discovery Using HPLC-Chip/ MS-Enabled ESI-TOF MS

Development of a novel site-specific mutagenesis assay using MALDI-ToF MS (SSMA-MS)

We have developed and validated a novel site-specific mutagenesis assay, termed SSMA-MS, which incorporates MALDI-ToF mass spectrometry (MALDI-MS) analysis as a means of determining the mutations induced by a single DNA adduct. The assay involves ligating an adducted deoxyoligonucleotide into supF containing pSP189 plasmid. The plasmid is transfected into human Ad293 kidney cells allowing repli...

HPLC-UV, MALDI-TOF-MS and ESI-MS/MS Analysis of the Mechlorethamine DNA Crosslink at a Cytosine-Cytosine Mismatch Pair

BACKGROUND Mechlorethamine [ClCH(2)CH(2)N(CH(3))CH(2)CH(2)Cl], a nitrogen mustard alkylating agent, has been proven to form a DNA interstrand crosslink at a cytosine-cytosine (C-C) mismatch pair using gel electrophoresis. However, the atomic connectivity of this unusual crosslink is unknown. METHODOLOGY/PRINCIPAL FINDINGS HPLC-UV, MALDI-TOF-MS, and ESI-MS/MS were used to determine the atomic ...